Clyde’s Calcium Assay features:
Ratiometric Dyes
Clyde’s CellOPTIQ® system uses ratiometric dyes to measure calciumWavelength Analysis
Analyses the ratio between the two wavelengthsRobust Readout
Obtains measurements unaffected by movement artefact or variations in cell layer thicknessSuperior Accuracy
Produces data that is more accurate, with significantly reduced signal-to-noiseSimultaneous Measurements
CellOPTIQ® measures simultaneous voltage and calcium at 10,000 times per second provides detailed information about their interaction in arrhythmic action potentials where EADs are present. Sequential measurement cannot provide this informationExample Data - Verapamil
Verapamil was used as an example of mixed action compound. Low concentrations of Verapamil (30nM), an antiarrhythmic drug with known mixed hERG and L-type Ca2+ channel activity, had no effect on APD (APD75 = 1.5 ± 0.7%) (Panel A), whereas the amplitude of Ca2+ transient was significantly reduced (45 ± 8%, p<0.05) (Panel B). This further highlights the need for measuring more than one parameter when testing compounds with mixed ion channel activity.
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